How to create a multiple sequence alignment?

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Katie A Burnette

You can get a good start using the free BLAST tools on Pubmed. That will get you an initial alignment, let you see if there are publications about your sequences, and pull up new sequences from the published database.

Ayoob Obaid Alfalahi

You can use the MEGA6 software, which can perform many statistical tests in addition to the sequence alignment, please see the attached file!