Hello everyone,
I was wondering what's your take on siRNA measurements in lipopolyplexes. We have recently tried chloroform: methanol extraction followed by invubation with 2% heparin and electrophoresis - it worked, but efficacy could be improved. Maybe someone tested other approaches? I have also checked disintegration with 1% Triton X-100 (on tRNA for training purposes) and appeard to be detrimental to the material. Maybe someone knows less troublesome method? I would appreciate your insights.