Michel Wehbe

4 Questions 5 Answers 0 Followers

Questions related from Michel Wehbe

How to create a multiple sequence alignment?

I am new to bioinformatic analysis of nucleotide sequences. I have several NGS nucleotide sequences (virus) coming from patients, that i need to align and perform a phylogenetic analysis. I would...

25 January 2018 6,820 3 View

How to align and construct a phylogenetic tree-rotavirus sequences?

Hello, my question is more directed to those who have experience in treating sequencing data: alignment and phylogenetic tree construction. I have ratovirus sequences coming for NGS (clean...

20 November 2017 10,044 2 View

TCID50 assay protocol?

Hello, I want to determine the infectious titer of an HSV strain on vero cells in a 96 wells plate. This is the first time i use the TCID50 test, so i have some basic questions: -how many cells do...

30 November 2016 5,557 3 View

Can you answer my question concerning the production of an infectious RNA virus form it's cDNA?

. I am trying to produce Enterovirus virions by transfection. At first I tried to transfect the transcribed viral RNA into HeLa cells with lipofectamine 2000, but I did not get any infectious...

05 September 2014 9,783 3 View