i want to create a stable cell line which have permanently lost their capability of expressing a certain protein. is this possible?
Of course it is. You are going to need some plasmids for knockdown or knockout, transfec into your cells and then screen using the resistance genes (such as puromycin, hygromycin B, etc).
Hi there,
It is possible provided the gene is not essential...
You can also use interfering RNAs as well as eliminating the gene. There are also conditional knockout systems available
Hello
yes , you can do that . also in this process you need use a plasmids such as shRNA . But we can help you more if we knew the type of cells .
pleas , see the attachment
Good Luck
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