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i have a membrane bound protein expressed by two different cell lines. i want to see if there are any mutations in the sequence of the protein. the simplest method i can think of is to isolate...
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i want to create a stable cell line which have permanently lost their capability of expressing a certain protein. is this possible?
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I've grown different cells wanting to assess caspase-8 levels in the whole cell lysate. I haven't treated them with anything. I'm unable to visualize caspase-8 in a western blot. What might be...
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