you can use the BLAST sequence alignment program that is incorporated in the NCBI webpage.
The following video from Science Buddies is a comprehensive guide on how to process the data you have collected and also on how to create your phylogenetic tree:
https://www.youtube.com/watch?v=WRKQGwh_Mw0
I hope that was helpful.
(PS: Many thanks to the creators of the video content)