Hello Dear Androniki,

First, you should download the genomic DNA data set of SWEET4 and CYP71B24 gene regions from NCBI in fasta format, then go to bash or powershell (you can use it in another command line interface) and add the downloaded genomic data to the header (for example: >NC_003074.8:c9599988-9597941). CYP71B24 ), you should create a new sequence file by pressing Ctrl+N in the Serial cloner program for both gene regions. Then, in the Primer3 program, you should paste these two sequences as only their nucleotide sequences and enter the sequence ids separately. You can run it with default settings, but you should remember that detailed settings must be changed from run to run. Once the program runs, it will give you 2 different primer sequences. You should go to the Serial Cloner program and create a new sequence file with Ctrl+N for these primer sequences and save their names as [related gene region]_left_primer.xdna and [related gene region]_right_primer.xdna. Afterwards, you should come to the toolbar above and click on the PCR option. You can name the displayed inputs, for example, SWEET4_left_primer and SWEET4_right_primer. Afterwards, you should click on the open option on the right side of the primer input and select the primers that you took from primer3 that you saved before and saved with .xdna extension in Serial cloner. The program will automatically detect the primers. Afterwards, you should select the xdna extension file you saved before from the Select Matrix DNA Sequence option. What you need to pay attention to here is to check whether the relevant primers are correct. You can then run it by clicking the Evaluate PCR and Run PCR options. I am also sending you additional data that will help you. I wish conveniences