Dear Sir,

I hope this message finds you well and in good health.

I am writing to seek your guidance regarding an issue I encountered during the annotation of my phage sequence using DNA Master. After performing the auto-annotation, DNA Master did not identify any tRNA sequences. Consequently, I used tRNAscan-SE to identify the tRNAs and manually added them into DNA Master by clicking on 'Insert' and entering the correct coordinates, followed by validating and reassigning the gene data.

After adding the tRNA sequences, I noticed that they appeared at the bottom of the list. When I sorted the sequences by 'Start,' the tRNAs moved to their correct positions in the sequence. However, when I exported the feature table file, the tRNA sequences were still listed at the bottom.

My question is, when submitting the file to NCBI, should the tRNA sequences appear at the bottom, or should they be in the correct order according to their sequence positions? If they need to be in the correct order, could you please advise me on how to achieve this?

Thank you for your assistance. I look forward to your kind reply.

Best regards,