How to evaluate the concentration of the standard preparation against an independent standard preparation?

Kindly assist about the evaluation of the concentration of the standard preparation against an independent standard preparation, prior to determine the selected sample calibration curve graph.

08 September 2018 4,216 0 View

Please educate me, What potential risks if my sample solvent is stronger than my mobile phase in HPLC analysis?

Professional advise will be appreciated, What potential risks if my sample solvent is stronger than my mobile phase in HPLC analysis? What happen or causes and solution

07 August 2018 1,889 0 View

What is most recommended samples purification between Column chromatography and Preparative Layer Plates (PLC) for HPLC analysis?

Kindly help with details advantages of Column chromatography over Preparative Layer Plates and disadvantages or vice versa for HPLC analysis

06 July 2018 8,688 3 View

What is the recommended procedure to clean the HPLC column daily before and after used?

Kindly help the recommended procedure to clean the HPLC column daily before and after used

06 July 2018 1,880 2 View

Which method of separation is more preferable, HPLC, NMR or GC for the determination of diatereoselectivity in polar or non-polar aldol products?

Which method of separation is more preferable, HPLC, NMR or GC for the determination of diatereoselectivity in polar or non-polar aldol products. What are the advantages or disadvantages of HPLC...

05 June 2018 7,753 9 View

What is simple methodology for the determination of diastereoselectivity by H NMR in the reaction mixture?

I need comprehensive procedure for the determination of diastereoselectivity by H NMR in the reaction mixture?

05 June 2018 7,161 2 View

Problem with ChiralPack IB column, can anyone help with best cleaning procedure?

Please, I have problems with ChiralPack IB column, can anyone suggest any possible solution because having poor baseline and can't find corresponding peaks.

01 January 1970 8,562 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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03 March 2021 5,360 2 View