How to evaluate the concentration of the standard preparation against an independent standard preparation?

Kindly assist about the evaluation of the concentration of the standard preparation against an independent standard preparation, prior to determine the selected sample calibration curve graph.

08 September 2018 4,216 0 View

How to choose column for HPLC analysis?

Please advice on best method approach to choose suitable column for HPLC to analyze targeted samples.

07 August 2018 4,700 0 View

Please educate me, What potential risks if my sample solvent is stronger than my mobile phase in HPLC analysis?

Professional advise will be appreciated, What potential risks if my sample solvent is stronger than my mobile phase in HPLC analysis? What happen or causes and solution

07 August 2018 1,889 0 View

What is most recommended samples purification between Column chromatography and Preparative Layer Plates (PLC) for HPLC analysis?

Kindly help with details advantages of Column chromatography over Preparative Layer Plates and disadvantages or vice versa for HPLC analysis

06 July 2018 8,688 3 View

What is the recommended procedure to clean the HPLC column daily before and after used?

Kindly help the recommended procedure to clean the HPLC column daily before and after used

06 July 2018 1,880 2 View

Which method of separation is more preferable, HPLC, NMR or GC for the determination of diatereoselectivity in polar or non-polar aldol products?

Which method of separation is more preferable, HPLC, NMR or GC for the determination of diatereoselectivity in polar or non-polar aldol products. What are the advantages or disadvantages of HPLC...

05 June 2018 7,753 9 View

What is simple methodology for the determination of diastereoselectivity by H NMR in the reaction mixture?

I need comprehensive procedure for the determination of diastereoselectivity by H NMR in the reaction mixture?

05 June 2018 7,161 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

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03 March 2021 5,360 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View