Kindly help the recommended procedure to clean the HPLC column daily before and after used
Mehrdad Rabbani
Hi Isiaka
if you use C18 column maybe this instruction could be helpful for you:
- For short term storage, i.e. over night, columns can be stored in the eluent used in last
analysis.
- For middle term storage, i.e. 2 days or over the weekend, columns should be flushed
with pure water to prevent microbial growth.
- For long term storage, silica based columns should be stored in an aprotic solvent.
The water content should not be higher than 50%. The best storing solvent is
Acetonitrile.
and other notes about caring the HPLC column provided in file that I atached. I hope it will be useful for you.
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