How to choose column for HPLC analysis?

Please advice on best method approach to choose suitable column for HPLC to analyze targeted samples.

07 August 2018 4,700 0 View

Please educate me, What potential risks if my sample solvent is stronger than my mobile phase in HPLC analysis?

Professional advise will be appreciated, What potential risks if my sample solvent is stronger than my mobile phase in HPLC analysis? What happen or causes and solution

07 August 2018 1,889 0 View

What is most recommended samples purification between Column chromatography and Preparative Layer Plates (PLC) for HPLC analysis?

Kindly help with details advantages of Column chromatography over Preparative Layer Plates and disadvantages or vice versa for HPLC analysis

06 July 2018 8,688 3 View

What is the recommended procedure to clean the HPLC column daily before and after used?

Kindly help the recommended procedure to clean the HPLC column daily before and after used

06 July 2018 1,880 2 View

Which method of separation is more preferable, HPLC, NMR or GC for the determination of diatereoselectivity in polar or non-polar aldol products?

Which method of separation is more preferable, HPLC, NMR or GC for the determination of diatereoselectivity in polar or non-polar aldol products. What are the advantages or disadvantages of HPLC...

05 June 2018 7,753 9 View

What is simple methodology for the determination of diastereoselectivity by H NMR in the reaction mixture?

I need comprehensive procedure for the determination of diastereoselectivity by H NMR in the reaction mixture?

05 June 2018 7,161 2 View

Problem with ChiralPack IB column, can anyone help with best cleaning procedure?

Please, I have problems with ChiralPack IB column, can anyone suggest any possible solution because having poor baseline and can't find corresponding peaks.

01 January 1970 8,562 0 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Can I use a graph from a another source in a thesis?

I have a permission from X company to use their graph from a report, but they have a reference in the bottom left corner of the graph as follows: "Source: Database Y". Is it OK to use this graph...

24 February 2021 2,269 3 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Could you please suggest to me research in which a relation between the shear wave velocity and the degree of saturation for stone was discussed?

Hi all, I am looking for a graph or a relation that relates the degree of saturation and the shear wave velocity for the marlstone. Looking forward to your suggestions Best Regards

24 February 2021 9,690 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,351 2 View