Anoxic protein purification - Impact of sonication on Fe-S cluster proteins?

Dear all, I need to purify anoxically a protein with Fe-S clusters (sensitive to oxygen). I used to do cell lysis using a French press but because the instrument is too big to be place in my...

08 November 2020 9,528 3 View

How to compute the effects in half fractions?

Greetings, I'm doing a screening design and want to allow for lack of fit. I need replications so I want to add four additional points to my dataset. Rather than use center runs I use corner...

11 October 2020 9,672 1 View

Meta-analysis in Stata : how to perform it with effect sizes ?

19 December 2019 9,553 2 View

How to find 3 initial parameters for gompertz curve in presence of other predictors

Given the following when fitting a three parameter gompertz curve to a predictor xg(x) = alpha * exp(- gamma * exp(- beta *x) ) I need to know whether gamma is equal to the inflection point of...

13 May 2017 2,803 11 View

How to find initial parms estimates when doing nonlinear analysis?

proc NLIN data = eleven BEST = 10 MAXITER = 100 METHOD = Gauss CONVERGE=1.0E-6 LIST ALPHA = 0.05 OUTEST = outest(where = (_TYPE_ = "COVB")); parms B1 = 1 B2 = .5128 B3=-.0036; model y =...

26 March 2017 2,174 5 View

Does anyone know how to extract with Matlab the georeferencing information of a JP2 image?

I'd like to read with Matlab the georeferencing metadata contained in a JP2 image. Any idea ? Thanks !

18 June 2015 859 4 View

What is the best housekeeping gene for a Western blot on purified adipocytes? GAPDH disappears after the purification.

Western blot

19 December 2012 6,810 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View