Anoxic protein purification - Impact of sonication on Fe-S cluster proteins?

Dear all, I need to purify anoxically a protein with Fe-S clusters (sensitive to oxygen). I used to do cell lysis using a French press but because the instrument is too big to be place in my...

08 November 2020 9,528 3 View

How to compute the effects in half fractions?

Greetings, I'm doing a screening design and want to allow for lack of fit. I need replications so I want to add four additional points to my dataset. Rather than use center runs I use corner...

11 October 2020 9,672 1 View

How to choose an ID variable in the HLM software student version ?

When I have to choose my identification variable in lvl 1 and lvl 2, I cannot choose it because it is grayed out. Do you know why ? and how to fix it?

27 March 2020 8,558 2 View

Meta-analysis in Stata : how to perform it with effect sizes ?

19 December 2019 9,553 2 View

How to find 3 initial parameters for gompertz curve in presence of other predictors

Given the following when fitting a three parameter gompertz curve to a predictor xg(x) = alpha * exp(- gamma * exp(- beta *x) ) I need to know whether gamma is equal to the inflection point of...

13 May 2017 2,803 11 View

How to find initial parms estimates when doing nonlinear analysis?

proc NLIN data = eleven BEST = 10 MAXITER = 100 METHOD = Gauss CONVERGE=1.0E-6 LIST ALPHA = 0.05 OUTEST = outest(where = (_TYPE_ = "COVB")); parms B1 = 1 B2 = .5128 B3=-.0036; model y =...

26 March 2017 2,174 5 View

Does anyone know how to extract with Matlab the georeferencing information of a JP2 image?

I'd like to read with Matlab the georeferencing metadata contained in a JP2 image. Any idea ? Thanks !

18 June 2015 859 4 View

What is the best housekeeping gene for a Western blot on purified adipocytes? GAPDH disappears after the purification.

Western blot

19 December 2012 6,810 5 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View