Hello everyone,
I am developping a TCID50 test for CMV virus, cytopathic effect should appear 5 days after inoculation. I wonder if it's possible to maintain MRC-5 cells at a concentration of 5.104/100µl in a 96 well plate for so long?
Do I need to change my maintenance media (MEM-98-SVF 2%)?
Plus, does anyone know if CMV's cytopathic effect can be easily visualized in 96 well plate ? (I was thinking to stain my cells at the end with erytrosine).
Thanks you for your help,
Best regards
Marion