I am mainly wondering whether the intensity is strong enough and how long a time course (tumor cell line) is feasible.
Research being conducted by Stellenbosch University would like your opinion about the effects of social exclusion on emerging urban mobility options and traditional public transport services. By...
21 July 2024 4,032 0 View
I will be administering 5-fluorouracil at a dosage of 50 mg/kg to mice as an I/P injection. Will someone please check my calculation? (Mw: 130.078 g/mol) Max volume to be administered to mice I/P...
23 June 2024 3,847 1 View
Dear Survey Experts, I am currently researching trust and resilience in the supply chain and seeking efficient ways to find survey respondents. In addition to social media and business...
21 June 2024 395 2 View
Hi! I have a question. I was looking into the research question: does mohs micrographic surgery give less recurrences and metastasis than standard excision in the treatment of cuteanous squamous...
17 June 2024 7,769 5 View
I have porous PMMA microparticles up to 1mm in diameter. I would like to functionalise the surface with amines whilst keeping the particles intact. Chat GTP has recommended ethanolamine or ATPES...
13 June 2024 7,977 1 View
Hi, I have been using a Q-Sonica Q500 Sonicator to disperse samples on nanocellulose and make emulsions. I have notices black dust like particulates settling out in some samples and on the bottom...
12 June 2024 9,737 6 View
I'm working on the RAFT copolymerization of MPC and NIPAm monomer, but I'm not sure how to calculate the total conversion from proton NMR? Is it an average of conversions of two monomers or just...
10 June 2024 7,430 2 View
Dear ResearchGate, I came across the ResearchGate page of Adri Jacobs, who passed away in 2010. This page contains 25 publication of Adri mainly from the period 2000-2008. One paper (2011) must...
01 June 2024 8,763 1 View
I am looking for resources in the Netherlands regarding cultural tourism. So what cultural activities correlate, and what lifestyles can be distinguished. Any resource is welcome, articles, factor...
27 May 2024 447 2 View
I've coated plastic water bottles (PET) with Nile Red to detect the plastic degradation by Ideonella sakaiensis. When using the settings for Sypro Ruby, the coated and uncoated plastic looks the...
23 May 2024 3,364 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View