Anoxic protein purification - Impact of sonication on Fe-S cluster proteins?

Dear all, I need to purify anoxically a protein with Fe-S clusters (sensitive to oxygen). I used to do cell lysis using a French press but because the instrument is too big to be place in my...

08 November 2020 9,528 3 View

How to compute the effects in half fractions?

Greetings, I'm doing a screening design and want to allow for lack of fit. I need replications so I want to add four additional points to my dataset. Rather than use center runs I use corner...

11 October 2020 9,672 1 View

How to choose an ID variable in the HLM software student version ?

When I have to choose my identification variable in lvl 1 and lvl 2, I cannot choose it because it is grayed out. Do you know why ? and how to fix it?

27 March 2020 8,558 2 View

Meta-analysis in Stata : how to perform it with effect sizes ?

19 December 2019 9,553 2 View

How to find 3 initial parameters for gompertz curve in presence of other predictors

Given the following when fitting a three parameter gompertz curve to a predictor xg(x) = alpha * exp(- gamma * exp(- beta *x) ) I need to know whether gamma is equal to the inflection point of...

13 May 2017 2,803 11 View

How to find initial parms estimates when doing nonlinear analysis?

proc NLIN data = eleven BEST = 10 MAXITER = 100 METHOD = Gauss CONVERGE=1.0E-6 LIST ALPHA = 0.05 OUTEST = outest(where = (_TYPE_ = "COVB")); parms B1 = 1 B2 = .5128 B3=-.0036; model y =...

26 March 2017 2,174 5 View

Does anyone know how to extract with Matlab the georeferencing information of a JP2 image?

I'd like to read with Matlab the georeferencing metadata contained in a JP2 image. Any idea ? Thanks !

18 June 2015 859 4 View

What is the best housekeeping gene for a Western blot on purified adipocytes? GAPDH disappears after the purification.

Western blot

19 December 2012 6,810 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

No title

02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View