I'm trying to isolate pure compounds from hexane extract of a plant bark. After VLC, CC, and SEC, I ran 2D-TLC (40% EtOAC in hexane) but the compound assumed to be as a mixture with another compound is still making tails after many separating techniques. Please let me know how to confirm whether the comound tails bcoz of it's phenolic property or is it an another compound runs with same Rf of previous compound or any other degradation scenario?
Thank you..
Hi, to prove whether is stable or not, please read page 2 of the attached publication, best regards
Hi.
May be you should consider adjusting the length of the column as well as the time-frame for collection of fractions. As for the tailing, it may be an indication of a new compound. This so because same Rf value alone, does not infer that the compounds can not be different.
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