Umapriyatharshini Rajagopalan

5 Questions 8 Answers 0 Followers

Questions related from Umapriyatharshini Rajagopalan

How to check whether the compound is degrading on Silica TLC plate or not?

I'm trying to isolate pure compounds from hexane extract of a plant bark. After VLC, CC, and SEC, I ran 2D-TLC (40% EtOAC in hexane) but the compound assumed to be as a mixture with another...

11 October 2016 3,521 3 View

How can I find the gene expression level of a single disease gene in normal and diseased individuals?

I wanted to find the gene expression level of BEST1 gene (responsible for Best disease - occur in eye) in normal and diseased individuals or cells using GEO Omnibus - NCBI, but I don't know how to...

22 May 2014 6,451 4 View

How do prions become infectious, do they can produce their own copies in multiples? Are they living things or any other new forms?

Prions are dangerous misfolded infectious proteins - which I read in an article about mad cow disease. Up until prions I used to think, only nucleic acids could be replicated into this earth. How...

10 April 2014 9,505 14 View

Can anybody suggest an effective media to culture Saccharomyces pastorianus?

I try to optimize the conditions for both liquid cultures (in Bio reactors) and agar cultures.

27 March 2013 4,725 3 View

In which ratio/concentration I should add IPTG+X-gal to ger blu-white colonies?

I tried to prepare blue-white colonies to check the tranformation efficiency, but coldn't get enough observation with my E.coli plates. I added IPTG and X-gal into a vial and then aliquot it and...

26 February 2013 5,264 2 View