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I ordered two Predesigned assays for quantification of CpG methylation by Pyrosequencing from Qiagen and I do not have any product after PCR. I have dissolved them in TE ph8, tried different...

22 September 2020 3,328 6 View

What does it mean to see a band for uncloned vector and one band for vector with insert from one colony after cloning?

I used gateway cloning to clone my insert into the donor vector. After having colonies, I did a mini prep and ran it on the gel. On the gel I could see a clear band on the right length for my...

14 June 2020 9,829 3 View

What are the soil nutrients use efficiencies in Central America?

Hello everyone, I am working in Central America and I would like to create a document that could help me to calculate "simply" the nutrients disponibility in the soil of coffee fields. The...

10 June 2020 9,671 4 View

How to perform gradient qPCR with Roche Light Cycler 480, to determine the best annealing temperature of my primers??

I would like to determine the best annealing temperature for my primers, giving the lowest Cq and the best efficiency. Is there a thermal gradient feature on the Roche LC480 instrument? And if so,...

16 February 2020 9,471 3 View

Which statistical analysis should I use for 2 dependent variables that are measured with a Likert Scale?

I am studying the generational changes in the relationship between empathy and social media usage. I am using a Likert Scale to measure both empathy levels and social media usage; however, I do...

20 January 2020 9,617 4 View

Is there a way to statistically separate the top percent of performers in a pre-test condition, to then analyse their scores in a post-test measure?

Essentially in a study where there is a pre-test, then an intervention and a post-test (Identical tests), I have a question: "Do students who perform better on the skill test before the...

12 December 2019 9,096 4 View

Why are my re-probings for Western blot analysis unsuccessful?

Hello everyone, I am having problems with re-probing membranes for Western blot analysis. Almost every time I want to re-probe with either the same or a new primary antibody, I don't get any...

11 December 2019 5,066 5 View

How to dissolve and inject 4-hydroxytamoxifen to nude mouse?

What is the normal way to inject 500 ug 4-hydroxytamoxifen per mouse? Is it dissolved 4-OHT in ethanol and diluted with peanut oil at least 10 times?

10 May 2019 3,301 1 View

Why does this strange situation occur in my nude mice xenograft after drug injection?

I injected cancer cells to mice as xenografts. After 7 days, I injected tamoxifen together with an inhibitor of interleukin to nude mice subcutaneously. But 2 days later, the tumor site turned to...

21 March 2019 6,082 4 View

Does anyone have experience in cleaving Fibronectin using an MMP before Western Blot analysis?

We are trying to demonstrate the our treatment effects increases the activity of MMP-2 by showing mutiple cleaved fibronectin bands in a western blot? For this we are going to have a control of...

13 February 2019 7,734 3 View

Which Landsat image/data set of USGS Earth explore help to mapping soil types in ArcGIS software?

for a large scale, ecological studies it's very important to distinguish between homogeneity and heterogeneity within an area.

15 November 2020 7,791 3 View

What stats is best for meta analysis on the prevalence of a disease and how to assess its heterogeneity?

Hi! Me and my team mates are currently doing a meta analysis on the prevalence of cytokine elevation and its correlation to the severity of a disease. Considering that we will be using cohort...

03 November 2020 6,867 4 View

How to perform Subgroup analysis in meta-analysis by using R ?

I am conducting meta-analysis by using R package (metafor), it seems i have a significant heterogeneity, so I need to do sub-group analysis guided by gender (categorical) and age (continuous)...

18 September 2020 918 3 View

On considering the effect of homogeneous-heterogeneous reaction in fluid flow problem, what are the chemical species A and B?

31 August 2020 9,983 5 View

Heterogeneity in a meta-analysis?

At what level, one should accept heterogeneity in a meta-analysis. What are possible calculation/test, if we get heterogeneity.

01 June 2020 9,396 6 View

Can you use the FreeRate model for heterogeneity across sites in MrBayes?

Using IQTREE I received the most optimum models for my data to analyse phylogenetic tree-reconstruction. IQTREE is able to use, aside from the Gamma distribution for heterogeneity across sites...

31 May 2020 2,352 4 View

What is the best alternative test to ANOVA when both normality and homogeneity of variances are violated?

I am analyzing school data for a district that is mostly white but with native, black, and multiracial minorities. I'm seeking to determine whether there are statistically significant differences...

26 May 2020 7,006 7 View

Sampling size for plant species diversity study?

If my goal of vegetation sampling is to determine plant species diversity of a heterogeneous area (eg. lowland, montane), what is the appropriate sampling deign and how to determine sampling size?

02 March 2020 852 6 View

Sampling size for plant species diversity study?

If my goal of vegetation sampling is to determine plant species diversity of a heterogeneous area (eg. lowland, montane), what is the appropriate sampling deign and how to determine sampling size?

02 March 2020 5,162 4 View

How to check the homogeneity of variance in large sample size with 6000 variables?

I use R to do univariate regressions for a large data set consisting of 6000 variables. I would like to know whether checking the homogeneity of variance is necessary for large sample or not....

20 February 2020 5,989 3 View