Hi, I'm trying to knockout one gene in murine PDAC cell lines. However, I found the knockout efficiency is only about 70% (by gDNA sequencing analysis). Because it's not a good idea to pick single clones because of high heterogeneity of murine PDAC cancer lines. Is there any good method to get relatively high-purity (perhaps>90%) murine KO lines? PS, I have tried a lot of sequencing and cannot improve by sequence optimization.
To obtain high-purity murine (mouse) knockout (KO) cells, you typically need to follow a series of steps involving genetic manipulation, cell culture, and purification techniques. Here's a general outline of the process:
By following these steps, you can obtain high-purity murine KO cells suitable for various downstream applications in research, including the study of gene function, disease modeling, and drug discovery.
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