How to carry out multiple enzyme screenings at a time?

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What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

Curcumin encapsulation in Bovine Serum albumin (BSA)?

Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...

23 June 2024 8,785 5 View

How does a Man-in-the-Middle (MitM) attack work in the context of Transport Layer Security (TLS), and what specific mechanisms can be employed ?

Context In the realm of cybersecurity, Transport Layer Security (TLS) is widely used to secure communications over a computer network. Despite its robust encryption mechanisms, TLS is still...

19 June 2024 6,595 3 View

Why is colony PCR not working ?

I've been doing colony PCR for last few days but haven't seen any bands on the gel. I've tried both positive and negative controls, but I'm still not getting any bands. Here's my procedure: I use...

07 June 2024 203 2 View

Albumin Nanoparticle Synthesis, troubleshooting?

Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...

04 June 2024 1,501 0 View

If my standard quercetin equation is Y = 0.0057x + 0.0635 then how can I know the value of ethanolic extract of 10 mg/ml if absorbance value is 0.39?

I wanted to know the value of total flavonoid content in mgQE/gm. Total volume I kept in well plate is 200 µl in which extract was 20 µl. Anyone please help me with this problem

10 May 2024 5,658 4 View

What will be the value of potassium content in mg/100g if absorbance value is 45 ppm in flame photometer?

Amount of fruit powder used 0.15g; final volume of the extract was 100 ml and I have done my experiment in flame photometer. I did not know how to convert 45ppm in mg/100g. Please help me?

10 May 2024 3,173 0 View

Difficulty in Phase separation ?

Hey all I intend to isolate free circulating SiRNA in media using chloroform extraction. I have added chloroform to media containing SiRNA to get the phase separated. The Chloroform came in the...

17 April 2024 8,522 3 View

Suggestions required for use of Ion exchange resin?

For purification of carbohydrases (carbohydrate degrading enzymes) from bacterial cell free supernatant (CFS), which Ion exchange resin can be used? Are there prepacked columns available for the...

14 April 2024 3,699 0 View

Question: Herbicide formulations ?

Which is probably a better and efficient formulation for the application of Pre-emergent herbicide? A) Dry formulation B) Liquid formulation?

11 April 2024 5,157 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Technology developments may it for whatever reasons developed eventually its benefits are multi-Disciplinatary?

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28 July 2024 2,822 2 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View