Ahmed Elsadek Fakhr

6 Questions 39 Answers 0 Followers

Questions related from Ahmed Elsadek Fakhr

Why vitek II give resistant for cefipime though MIC less than 0.1?

Good morning all. I got a strain of Proteus mirabilis on Vitek II and it was an XDR strain. The cefepime has < 1 result but the machine made a star and consider as resistant. Manually it is...

16 October 2021 7,407 0 View

Is it necessary to dilute microRNA before Real time PCR?

I worked on microRNA and after transcription into cDNA and going into Real time PCR, I used different concentrations of cDNA. I noticed that diluted samples give better results and lower CT in...

15 July 2021 6,484 4 View

Have you an idea what this wonderful mold fungus is?

This mold is isolated from a case of sinusitis. the growth is after 4-5 days in room temperature The microscopic picture mostly for Asperigellus species. But it is not clear to me what is this...

07 July 2021 2,745 7 View

How to optimize concentrations for a custom made primer probe for sequence detection?

I got a custom made sequence detection primers with 10 nm scale and a probe with 6 nm scale for a microbial sequence and I did not get anything else from the company. I want to know how to set the...

20 December 2020 5,150 1 View

Is it possible to have a gram negative organism sensitive to gentamycin but resistant to amikacin?

In our lab. work, I met some times gram negative bacteria which is sensitive against gentamycin but amikacin resistant and manually the same thing. What is the interpretation of such cases or...

07 November 2020 1,537 6 View

If I kept my Real time PCR reagents in -80 instead of -20 can be affected ?

I have a problem in - 20 fridge and I have to keep my reagents in -80 Can it be affected if used daily for a time?

19 April 2020 8,794 8 View