Ahmed Elsadek Fakhr

5 Questions 34 Answers 0 Followers

Questions related from Ahmed Elsadek Fakhr

Why vitek II give resistant for cefipime though MIC less than 0.1?

Good morning all. I got a strain of Proteus mirabilis on Vitek II and it was an XDR strain. The cefepime has < 1 result but the machine made a star and consider as resistant. Manually it is...

16 October 2021 7,396 0 View

Is it necessary to dilute microRNA before Real time PCR?

I worked on microRNA and after transcription into cDNA and going into Real time PCR, I used different concentrations of cDNA. I noticed that diluted samples give better results and lower CT in...

15 July 2021 6,470 4 View

Have you an idea what this wonderful mold fungus is?

This mold is isolated from a case of sinusitis. the growth is after 4-5 days in room temperature The microscopic picture mostly for Asperigellus species. But it is not clear to me what is this...

07 July 2021 2,733 7 View

How to optimize concentrations for a custom made primer probe for sequence detection?

I got a custom made sequence detection primers with 10 nm scale and a probe with 6 nm scale for a microbial sequence and I did not get anything else from the company. I want to know how to set the...

20 December 2020 5,131 1 View

If I kept my Real time PCR reagents in -80 instead of -20 can be affected ?

I have a problem in - 20 fridge and I have to keep my reagents in -80 Can it be affected if used daily for a time?

19 April 2020 8,777 8 View