I have an inhibitor which was diluted in 100% DMSO, the stock concentration is 50mg/mL and the stock volume is 200µL. these were diluted in ddH2O to reach 10µM.
the question is how to calculate the final concentration of DMSO ?
For ionic liquids using electroneutral approach you have the option to define ionic liquid phase (1 mol cation: 1 mol anion), but what about deep eutectic solvent is it: 1 mol cation: 1 mol...
18 January 2023 3,708 0 View
I have stimulated thp-1 cells and applied inhibitor but the inhibitor did not work and I am wondering why would this coul happened, could it be because of the FBS ?
23 May 2022 763 1 View
Can someone help me by solving this issue? I have a cell culture of THP1 cells and I need to extract RNA but the cells are frozen and they have been washed by PBS and 0.1 M BSA is added. The RNA...
10 March 2022 925 2 View
Hello What is the best way to convert DPD powder (chlorine test) to tablets? Thank you for your help
03 December 2020 7,018 2 View
I want to research on aggressive and dangerous driving behavior. first I want to know does an aggressive driver necessarily dangerous driving? I mean if one person shows aggressive behavior, I can...
26 December 2019 7,780 3 View
1. Crucifers of Great Britain and Irland 2. Light and scanning electron microscopic studies on trichomes of some Brassicaceae[1983]
24 April 2019 215 1 View
Some business angel networks (BANs) specially in developing countries have very few investments, what are the challenges that they face in increasing their investments?
29 March 2017 4,604 2 View
I tried various functions in matlab but non of them could distinguish the boundary of the cross sections in the image.is any one to help? so thanks
29 April 2015 5,418 3 View
Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!
10 August 2024 857 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I have reached out to the original creator Dr. Demerouti but have had no luck with a response. I am completing my IRB form and need the information or permission by tomorrow. I know short notice,...
29 July 2024 3,684 0 View
What can be a hypothesis related to the Stock out Cost or Shortage Cost?
29 July 2024 9,259 2 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,827 1 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
I need to plate SHSY-5Y cell into 35mm dish for the Patch-clamp technique, and the paper said I need to reach the cell confluency about 40-60% . How many cells should I plate? And in advance, I...
04 July 2024 1,954 1 View