Dear folks, culture lactobacillus what is the parameter to maintain during fermentation batch size is 15L, which medium is suitable for lactobacillus and how to scale up the culture please share your knowledge .
Thank you.
Hi@ Lala K Nagaraj,
I hope you already carried out basic experiments with your organism with flask, either static or shake culture. Basically, for Lb species, MRS or YPG nedium will do. For culturing in a fermentor, you better apply your basic data obtained through the flask culture. For example, which medium your Lb sp. prefers, or if it grows better in static culture than shake culture, etc. meaning that it does not like/need too much oxygen, or the other, so, depending on your basic results, you have to control your aeration rate or agitation rate. So, better you carry out some flask cultures to learn the character of organism, and apply these data to perform a fermentation culture. Good luck.
Dear Lala,
As Tapan Kumar Mazumder said MRS for Lb species is the best way to carry out basic experiments. Using the best condition in MRS (lab scale) you can scale-up it to bioreactor. You can prepare it using the folowing reactives:
Peptone proteose................................10,00
Meat extract.......................................8,00
Yeast extract......................................4,00
D(+)-Glucose...................................... 20,00
Sodium acetate...................................5,00
Triammonium citrate.............................2,00
Magnesium sulfate...............................0,20
Manganese sulfate.............................. 0,05
Dipotassium phosphate........................ 2,00
Polysorbate 80....................................1,00
Final pH 6,2 ± 0,2 at 25ºC
Regards,
This is an open question , you have to specify the sp or at least the group of lactobacilli
Tapan Kumar Mazumder thanks for your reply already we obtimised mrs medium and temp maintained 37
wat is gyb medium
Hi, Lala K Nagaraj,
Thanks for your reply YPG medium is Yeast extracr, peptone & and glucose are the main components, besides other minor ones. Good luck.
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