Problem with PCR using plasmid as template?

Hi I am trying to do a PCR for a 1kb fragment using a plasmid of 11kb as a template. But unfortunately on the gel, the band is as large as the plasmid, that is 11kb. Would anyone know that that...

20 October 2020 7,631 3 View

How can I access the international articles related to Crop Monitoring?

I am planning build my career in agricultural development using remote sensing. Now I started surfing through articles for gaining knowledge. Could anyone can suggest me some articles?

25 August 2020 2,345 7 View

How the saturation temperature is to be provided in evaporation condensation model in ANSYS FLUENT?

I am planning to simulate condensation of moist air in ANSYS FLUENT. Say the inlet conditions of air is at 30 degree Celsius and relative humidity 80%. While giving mass transfer mechanism in...

09 August 2020 7,744 3 View

How to give relative humidity input in ANSYS FLUENT?

I am trying to simulate the condensation of moist air in ANSYS FLUENT. Say, the inlet temperature of air is 30 degree Celsius with relative humidity 80%. I assume that species transport model is...

03 August 2020 7,778 2 View

How to give relative humidity input in ANSYS FLUENT?

I am trying to simulate the condensation of moist air in ANSYS FLUENT. Say, the inlet temperature of air is 30 degree Celsius with relative humidity 80%. I assume that species transport model is...

03 August 2020 211 3 View

Can anyone suggest a way to overcome recombination when amplifying a pcDNA3 plasmid containing human SCN9A CDS insert?

I need to amplify a pcDNA3 plasmid containing human SCN9A CDS insert (pcDNA3-SCN9A construct). The CDS is around 6 kb, and the total construct length is approximately 11.2 kb, which is a bit...

07 June 2020 2,134 2 View

Can any one provide me MATLAB Code Of Lyapunov exponent for a 3-D ODE ?

Hi all, I'm looking for the Lyapunov exponent code for a 3-D ODE System preferably either for lorentz system or Rossler system. Can any one provide me with the MATLAB code.

09 February 2020 2,732 3 View

Query regarding bioinformatics?

03 November 2019 1,653 3 View

How elementary particles acquire mass and charge?

Elementary particles have their own mass and charge. Mass is known to be acquired by the Higgs field. How charge is acquired by a particle?. Is both the phenomena are dependent?.

08 June 2019 9,722 13 View

Relation between NDVI and tree leaf quantity?

I am trying to correlate tree leaf fall and NDVI. So my doubt is can a change in NDVI over a temporal window indicate a direct proportional change in the leaf amount. Can such a relation be...

18 May 2019 6,297 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

How to troubleshoot Electrochromic Films and Electrochromic Devices?

I am trying to characterize an electrochromic system. The system uses ITO electrodes on glass, WO3 as the electrochromic film, and NiO as the ion storage layer. When I deposit NiO and WO3 and...

23 February 2021 9,592 2 View

How to improve the adhesion between germanium and silicon monoxide thin film layers using EBEAM evaporation?

I want to deposit multilayer coating of germanium and silicon monoxide on silicon wafer using ebeam evaporation?

21 February 2021 2,141 3 View

How can I measure the thickness of thin layers deposited on textured glass susbtrates?

I want to measure the thickness of aluminum oxide and titanium oxide layers deposited on textured glass substates. The layer thicknesses are typically below 100nm.

21 February 2021 5,465 4 View

How do I calculate absorption coefficient of my thin film if I dont know the thickness? Or how can I calculate the thickness of my sample?

I have done Uv-vis characterisation of my thin film and have received absorbance and transmittance data. Now I want to calculate the absorption coefficient but I dont know the thickness of my...

21 February 2021 7,148 8 View

How to construct different filler shape in MD simulations?

Is there any way to construct different initial filler shapes like triangle, square, rectangle, pillar, and tube in any simulation software.

20 February 2021 1,750 1 View

Can fractal dimension of thin film surfaces affects the electrons energy states?

As fractal dimension of a thin film surface implies the irregularity in the surface. Is it possible that the energy states of the electrons are also affected by the fractal dimension?

19 February 2021 5,509 3 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View