01 January 1970 1 8K Report

I am interested in calculating the HIV proviral load from the host genomic DNA using a house-keeping gene. What would be the best way for quantification without using a standard DNA template concentration?

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Can I use AgPath One-Step Rt-PCR reagents kit for direct amplification of DNA targets?

I would like to know if it is a common reagent kit for the target amplification of both DNA and RNA, or does it show amplification only for the RNA targets. Will the presence of DNA in the sample...

18 December 2023 7,062 2 View

Mechanism of crystal staining?

Where does the crystal violet bind to when added to live and dead cells? Which part of the live cells they bind to?

09 October 2023 5,577 0 View

Molecular weight of PEG using NMR?

How can we detect the molecular weight of PEG in NMR? Is it possible to detect the number of monomers in that? Thanks

22 June 2023 1,447 1 View

What are the alternatives for proteinase K permeabilization in TUNEL staining?

We have got InSituCellDeathDetectionKit_Roche for TUNEL staining assay in intestinal tissue sections. What are the alternatives for proteinase K permeabilization? Can we use trypsin instead and...

15 June 2023 1,496 2 View

Does anyone have a Healthcare based real-time dataset kindly give me the link or text me?

Does anyone have a Healthcare based real-time dataset kindly give me the link or text me?

12 June 2023 4,658 0 View

How to determine whether the PEG is having linear or branched structure?

Which technique or analytical method should be used to determine the nature of PEG (polyethylene glycol). How do we determine whether it is having branched monomers or linear monomers?

25 May 2023 4,228 3 View

Which method can be used to determine the length of monomer in PEG?

I need to detect the length of a monomer in PEG. Is there any method? Because i need to determine the Flory's radius. So, in order to carry out the same. I need to detect the monomer length. Is...

17 May 2023 6,096 0 View

Analysis of PEG- COOH with MALDI-TOF?

I need to analyse my sample using MALDI -TOF. I'm getting reference articles for the determination of PEG but, i couldn't able to find the relevant articles for PEG-COOH anlaysis. Can anyone...

10 May 2023 8,252 20 View

How to determine the length of PEG chain?

Which method can be used to determine the length of the PEG (Polyethylene glycol) chain which has been grafted on Nanoparticle?

09 April 2023 3,574 4 View

How to determine the grafting density of PEG on nanoformulation or nanoparticles?

I need to identify the type of PEG Coating confirmation on the nanoparticle. Since, there are two types of confirmation based on the grafting density of PEG, I would like to know how we can...

31 March 2023 8,065 0 View

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Are the apoptotic cells is positive for γH2AX ?

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Illustra™ MicroSpin™ G-25 columns what it is used for?

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