Which method can be used to determine the length of the PEG (Polyethylene glycol) chain which has been grafted on Nanoparticle?
Dear friend Janani S.K.
The length of the PEG chain grafted on a nanoparticle can be determined using various methods such as gel permeation chromatography (GPC), size exclusion chromatography (SEC), dynamic light scattering (DLS), nuclear magnetic resonance (NMR), and mass spectrometry (MS).
GPC and SEC are the most commonly used techniques to determine the size and molecular weight distribution of polymers, including PEG. In these methods, the PEG chains are separated based on their size and the elution time is correlated with the molecular weight of the PEG chain.
DLS can also be used to determine the size distribution of PEG-coated nanoparticles. By analyzing the fluctuations of the scattered light caused by Brownian motion of the particles, the size of the particles can be determined.
NMR can be used to determine the length of the PEG chain by analyzing the chemical shift and peak intensities of the PEG protons. This method provides accurate information about the PEG chain length and can also be used to determine the degree of PEGylation.
MS is another powerful technique that can be used to determine the molecular weight and structure of PEG. By analyzing the mass-to-charge ratio of the PEG molecule, the length of the PEG chain can be determined.
In conclusion, the choice of the method to determine the length of PEG chain depends on the specific requirements of the experiment, the size of the nanoparticle, and the availability of equipment and expertise.
Kaushik Shandilya Thank you for your valuable inputs.
As you have mentioned, I've already determined the size of the nanoparticle after the pegylation using DLS (Dynamic light scattering).
Since, I need to determine the exact size of the PEG in nm, I would like to know whether I can opt for AFM (Atomic force microscope). Kindly help me with this.
Thank you.
Dear all, both grafts length and density are to be considered. Please have a look at the following documents. My Regards
https://doi.org/10.1021/acs.analchem.9b00707
10.3390/molecules24112106
https://doi.org/10.1016/j.biomaterials.2005.07.006
10.3390/molecules24112106
https://doi.org/10.1002/polb.23260
Abdelkader BOUAZIZ Thank you sir. I wanted to know the length of the peg chain. In few of these articles they have found the effect of peg length and grafting density. But I'm exactly not getting the point how to determine the length of the peg chain. Since, I'm new to this topic, it would be great if you let me know how exactly I can determine the length of peg chain.
Further, I thought the H1 NMR would be a better option. But even then, I'm confused, how can we can determine the length of peg in NMR. I would like to know whether it is based on height of the peak or area under the curve. Kindly help me out with this.
Thank you.
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