Why do primary liver cells isolated from porcine not attaching to the plate?

I isolated primary liver cells from porcine by two step collagenase method. I am using RPMI 1640 medium for culturing the cells on collagen coated plates (collagen coating is done by myself). The...

07 October 2015 7,247 3 View

Has anyone tried immunopanning human astrocytes?

I want to isolate/purify astrocytes from a mixed population of neurons and glial cells etc. I tried sorting with GLAST beads but with no luck. If you have tried immunopanning and if it worked for...

11 February 2015 6,473 7 View

How does one calculate the binding energy in the case of chemical functionalization assisted by doping?

If we have chemical functionalisation assisted with doping in carbon nanostructures, how to calculate the binding energy, is the formula BE = BE(A+B+C)-BE(A)-BE(B)-BE(C) correct....?

29 September 2014 7,521 3 View

What is the control signal in controller design? How can I get the response of this control signal?

31 July 2014 4,477 5 View

Could anyone suggest steps to make a concentration of pure protein?

Protein is dissolved in larger volume of buffer (ammonium acetate). Thank you.

04 October 2013 10,014 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View