I´ve done an extraction of Coca Cola to get a higher caffeine concentration. Here is the protocol I've used:
1. 20 ml Coca Cola + 2 ml theobromine (2 mg/ml)
2. extraction with ethylacetate, drying with sodium sulfate + filtration after
3. get rid of ethylacetate with rotary evaporator
4. residue resuspended in 2 ml methanol
Now my question is how to calculate the concentration of theobromine in my extract? My problem is that after the evaporation there was no liquid (as I expected). So my extract was present as a solid residue. After this I resuspended in methanol. How can I calculate theobromine? I know that I have 0,18 mg/ml of theobromine in my Coca Cola but I don't know how to calculate the dilution with methanol.
Thanks for your help!
Theobromine is a solid. If all of the initial theobromine ends up in the organic phase and all the solid is dissolved in the 2 mL of ethanol, then theobromine should be concentrated to 2 mg/mL in ethanol.
If you used 2 ml of 2 mg/ml. Of theobromine you had 4 mg of theobromine. But since you made a série of operations you cannot assume that extraction efficiency wss 100%. But you friend the extract. If you assume that ALL solid os Only theobromine you can weight the extract and calylate the concentration in methanol. But i sugestão tô makes an analysis of the solid ir the methanol solution since parte of the mass can né other componentes added durinho the processing. If you Only want to know an Idea of concentration weight the solid before methanol addition and divide by tão (the mL of methanol added). Thaís Will the maximum concentration. Good Luck!!!
Hi,
You may use the web tool to calculate
https://www.alfa-chemistry.com/solution-dilution-calculator.htm
https://www.alfa-chemistry.com/mass-molarity-calculator.htm
This article discusses; "How To Calculate A Dilution" at the link. https://www.sciencecompany.com/How-To-Calculate-A-Dilution.aspx
- Badges
- Science method