How to calculate cryoprotectant concentration for formulation?

29 May 2017 0 7K Report

If it says 5% w/v does that mean for drying 20 ml formulation containing whatever lipid concentration or drug amount we need to add 1gm of let say mannitol.

Badges
Science topic

More Vibhu Rinwa's questions See All

How the value of surface roughness influence the slurry erosion of the substrate?

Respected Mates I Have deposited the Cr2O3 coating on the CA6NM turbine steel with D- gun spray method. The obtained value of RA Surface roughness is 3.3 +-0.1 after the coating. I want to...

24 October 2023 7,586 0 View

What is the method of fabrication of rods from Hydroxyapatite (HAP) nano powder?

Respected Researchers, I want to fabricate the rods from Hydroxyapatite ( HAP) nano powder. I want to develop these rods for the coating purpose by thermal spray process. 1. Can opt the thermal...

14 September 2021 3,461 3 View

What is the effect of morphology and size of the raw powder on the microhardness obtained in the coating?

Dear Researchers I have done a Ni-20TiO2-20Al2O3 coating on the CA6NM turbine steel by the Flame spray method. The microhardness obtained after coating was observed lower than the literature. I...

10 August 2020 2,556 4 View

Can 8 molar urea alone is capable of breaking the disulphide bonds in proteins during cell lysate preparation?

Actually i need to purify the his tagged proteins and check for the formation of disulphide bonds,for which I am using TCA and HU buffer without dtt or merceptoethanol

04 March 2019 7,671 2 View

What will happen to my digested vector if digestion is done for longer than 8 hours?

I am using EcoRI HF and Xho I with cut smart buffer? It's 100 ul reaction with 1.25 of each enzyme and around 1 ug of vector?

09 October 2018 1,069 1 View

Which type of tensile bond strength coating filure is better? Cohesive coating failure, glue failure or adhesive failure?

Dear researcher I have made a tensile bond test of Ni-4TiO2 and NI-TiO2-Al2O3 coating deposited by HVFS. The application is for protection against slurry erosion in hydro turbine. After tensile...

09 August 2018 1,050 2 View

What is the effect of impact angle on CA6NM turnine steel?

Dear researchers i tested CA6NM turbine steel for impact angle on slurry erosion jet rig. At 90 degree angle i am getting higher erosion rate whereas, at lower impact angle i am getting lower...

12 July 2018 2,659 2 View

ReRAM Measurement using Keithley 4200SCS?

I am working on RRAM devices in which Pulsed I-V characterization is improtant for SET/RESET Switching and Endurance of the device. I have two 4200 SMU, two 4210 HPSMU, two 4225 PMU with RPMs...

06 November 2017 6,975 6 View

Paste fomation of ti02 in ball mill

Dear all i was trying to produce Ti02 nano particles in high speed ball milling machine. my initial setting was 400 rpm 6:3 start stop time and ethanol and stearic acid were used as PCA. After 9...

07 August 2017 7,754 2 View

DLS Data interpretation

Dear Friends. I am new user of DLS Test for measuring the size of nano particles i have made by ball milling machine. i am attaching one of picture of my DLS result. plz tell me how to interpret...

01 August 2017 10,023 10 View

I am considering injecting cisplatin directly into an equine sarcoid tumour that is chronically refractive?

My case has been under treatment now for 3 years with little response. Now considering euthanasia. Last ditch is Cisplatin for dilution in iv infusion (100ml/ml in saline and mannitol). What can I...

05 August 2024 1,835 0 View

What does mannitol do in isolation buffer...?

I am preparing isolation buffer for mitochondrial isolation purpose. In protocol, it is given to add 225 mM sucrose and 75 mM mannitol for preparing isolation buffer. I do not have mannitol in my...

01 June 2024 5,936 1 View

Can Achromobacter grow on nitrogen free mannitol broth?

We isolated azotobacter from soil for cellulose production but 16srrna identified it's Achromobacter.

13 March 2024 9,352 0 View

Which medium is the best to be used for qualitative estimation of IAA production of PGPR?

As per the tiltle said, I want to qualitatively test how much IAA can be produced by some of the bacteria that I have just isolated from a soil sample. After culturing the soil bacteria in a...

03 December 2023 4,408 1 View

Can a selective medium such as "bacillus chromoselect agar" allow the growth of bacteria of the genus vibrio?

I bought a selective medium to isolate bacteria of the bacillus genus (Bacillus chromoselect agar). The colonies looked good and the color corresponded to the manufacturer's recommendations. These...

08 June 2021 202 3 View

Can we use Spray dried solid lipid nanoparticles for MTT assay?

I have synthesized Quercetin SLNs and spray dried them using Mannitol to avoid drug leakage. However, I need to perform MTT (cytotoxicity). Can I use the powder directly disperse it into media and...

08 January 2021 6,967 3 View

What is the function of sucrose or mannitol in buffer for cell lysis?

In the subcellular isolation experiment, sucrose and mannitol is present in buffers for resuspending cell pellet after centrifugation. For example, Isolation buffer (IB): 220 mM d-mannitol, 70 mM...

28 May 2020 4,910 3 View

Can anyone help identify this unknown bacteria?

I'm having extreme difficulty identifying this bacteria... here are the results of some of the tests I've performed. Any insight is greatly appreciated! TTC Motility: strongly motile Gram stain:...

04 March 2020 2,351 6 View

How to calculate for adding 10% of excipient into formulation?

Hi, I need to add 10% of mannitol to make my formulation into 5 mL in total. I'm currently confused on how the calculation work. Hope all of you can help me.

22 December 2019 946 5 View

What is the best ingredients to make ODT which contain arround 100 mg natural extract?

My formulation contains 100 mg natural extract which also use Mannitol and Dextrose as diluent (total amount is 650mg). I have issues during the process, for instance, the granules will stick into...

11 December 2019 4,690 3 View