Can 8 molar urea alone is capable of breaking the disulphide bonds in proteins during cell lysate preparation?

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Jasim Al-Saadi

8 M urea do not break S-S bond . Urea break hydrophobic and hydrogen bonds .If you need to break S-S bond you can use 2-ME or DTT.

Tsepo Lebiletsa Tsekoa

You will need a reducing agent in the buffer to break disulfide bonds. Add beta mercaptoethanol (2 - 5%) or dithiothreitol at ~50 - 100mM concentration.