Hi, I need to add 10% of mannitol to make my formulation into 5 mL in total. I'm currently confused on how the calculation work. Hope all of you can help me.
Dissolve 0.5gm of mannitol in 5 ml of solvent
Explanation = 0.5g x 20=10 gm
5 ml x 20=100 ml
10 gm in 100 ml means 10% solution
10% (w/v) = 10 g/100 mL
10 g / 100 mL* 5 mL = 0.5 g
Weigh 0.5g of mannitol and dissolve in 5 mL of solvent/solution.
10% mannitol solution mean 100l contain 10gm mannitol.
So,
1ml solution contain = 10/100 = 0.1 gm mannitol.
5ml solution contain = 0.1×5 = 0.5 gm mannitol
Add 80ml water into a beaker.
then add 10gm mannitol into the water.
stir until the solution is clear.
make up the volume to 100ml.
(you may need to warm up the water in order to dissolve the mannitol easily.)
stoichiometrically 10% Mannitol means 10 g in 100 mL (0.1 g / mL)
From the above stock take 5 ml.
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