How to calculate cooling effect intensity and cooling effect distance of all urban areas with greenery from satellite images (Sentinel, Landsat)?

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Analysis of MHC-I and II alleles with CNVs and unassigned loci?

I am working on a dataset of MHC-I and II alleles from a bird species sequenced with Illumina. We were not able to assign alleles to loci through MHC-typer as we were over the limit of 150 alleles...

15 July 2024 182 1 View

What is the best method to measure diameter of new vessels in tissue,e.g. spinal cord?

Hello! I would like to study newly formed vessels after spinal cord injury in rats. However, I do not know what is the most suitable method to check diameter or number of vessels and which...

01 July 2024 9,744 0 View

Comsol: Why does the color bar display a single value, even though there is a temperature gradient on the graph itself?

The attached file shows that the results contain different isotherms and colors indicating different temperatures, but on the scale all colors indicate the same temperature value. What could be...

04 December 2023 10,005 1 View

Interpretation of results from HepG2-Lucia AhR cells?

Hello, does anybody know, how to interpret this results, please? We measured AhR activation with HepG2 Lucia AhR cells, we used different concentrations of our compound and FICZ as an AhR...

09 May 2023 9,006 3 View

Is it possible to compare the obtained pitch value for cholesteric liquid crystal from wedge method with the image from polarizing optical microscopy?

I need to resolve the discrepancies in the pitch measurement from these two methods as from wedge cell measurements (distance between the grandjean lines) I determined the value around 12...

08 December 2022 4,875 3 View

Lisrel: My model does not converge. Why?

Hello, I would like to set up a path model (build Simplis syntax) using the Lisrel software, nevertheless, when I attempt to run a model, the system declares: the model does not converge and I...

16 July 2022 4,196 5 View

Paleolimnology. Please would anyone know what these objects are?

We find them frequently in sediment cores of an antarctic lake, they are fairly large (150 um) and we cannot identify them. Any idea are welcome! Thanks, Veronika

20 March 2022 2,244 5 View

Is it possible to distinguish male/female reindeer from a photo?

We want to split the population according to sex and measure certain body parameters separately. As both sexes have antlers unlike other ungulates, it is quite complicated...

18 January 2022 8,121 3 View

How to choose the size of FITC-dextran to determine mesothelial cell monolayer permeability?

I have to check the permeability of the mesothelial monolayer. I am using a 96-transwell plate with 8 μm pores, coated with type I collagen and then culturing the cells to form tight junctions and...

09 January 2022 6,281 1 View

What are the weird results/peaks of cDNA Library for RNASeq on TapeStation?

Hello, I am currently preparing cDNA Library with Nebnext Ultra II Directional RNA Library Prep Kit from exosomal RNA (patients with multiple myeloma). My research is centered around lncRNA. After...

30 October 2021 1,311 0 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

How to identify wetland area in Landsat imagery?

The indexes NDWI and MNDWI are use to identify waterbody. Can I use these index to identify wetland also? Thank you for advance.

25 July 2024 6,242 2 View

How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,770 1 View

What are the key risks associated with foodborne pathogens in imported leafy greens, the impact on public health and effect on regulatory framework?

I am currently writing a systematic review

15 July 2024 9,176 1 View

How do i go about my research which is about the integration of solar and wind power to produce green hydrogen?

Let me say I have a solar plant in operation and I want to build up a wind power plant, and later make a hybrid so that I could power the electrolyser as load for green hydrogen production.

14 July 2024 9,547 4 View

I need to connect expert in Urban design for Health ?

I studying PhD course in Urban design for Health. I need to know in some fileds: residental block, green open space and Transport TOD. I need connect with expert in that fileds.

14 July 2024 1,892 3 View

When Using SYBR Green for Flourescent Detection of LAMP Products what causes an Overshoot in Flourescence Reading?

The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65...

14 July 2024 1,483 0 View

Gulam Mohiuddin

The most convenient way would be calculating land surface temperature (LST) from Landsat data and assessing the temperature distribution in terms of green areas and their vicinity. You can follow our article to know more about LST: Article Application of Land Surface Temperature Analysis in Urban Gr...

Benjamin Beaumont

Dear Veronika, we have submitted a paper to IGARSS 2022 on the mapping of green infrastructure, mapping the current UHI situation in the city of Liège (Belgium), on which greening scenarios will be build by the authorities. Based on these, we will use a 1m model to map the impact of these planting scenarios on a predicted UHI. Link to a publication on the given model: https://www.mdpi.com/2225-1154/8/1/6

Veronika Murtinová

Thank you for all of your suggestions.