How should I deal with missing data?

12 February 2021 5,306 4 View

Athena IE in surrogate modeling?

I was wondering if anyone has deployed Athena IE in surrogate modeling or metamodels for LCA. There are many research involving data-based models for predicting energy consumption, but I haven't...

23 January 2021 2,267 1 View

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01 January 2021 9,970 2 View

How can I normalise cell count/protein concentration using SDS Coommasie blue stained gel?

Im quite new to cell culture and have had some inaccuracies while harvesting my cells and therefore counting them using a CASY counter. I reconstituted in what I thought to be 50,000 cells/ul,...

16 November 2020 9,829 1 View

Hazard value for malaria survival graph??

In an experiment untreated infected mice and infected treated mice observed. I recorded my data in terms of survived mice to 28 days in each group. How to calculate hazard value for treatment vs...

06 November 2020 5,548 1 View

What is the best blocking agent to block rGO biosensors?

I am trying to make a DNA sensor but I am detecting non specific binding. Does anyone know a better blocking agent for DNA sensors based on rGO, apart from BSA or MCH?

05 November 2020 5,140 3 View

No proteins of interest after Co-IP with Dynabeads?

Hello, I am trying to Co-IP my protein complex of interest, and every time i run it on the gel I get nothing but antibody bands. I conjugate my antibody to Dynabeads protein G overnight. I then...

29 October 2020 3,229 7 View

What's the best DNA electroporation program for immature dendritic cell ?

Hi what's the best DNA electroporation program for immature dendritic cell ?

17 September 2020 5,379 3 View

What kind of MMP should I use If I want to degrade rat tail collagen (Type I) gels?

I want to do MMP digestion on my collagen gels to see the effect of glycation on my collagen gels. I have difficulty to find the best MMP! some articles used MMP-1 some MMP-9 some MMP -2 and some...

28 July 2020 6,711 1 View

How can i test the Security for an algorithm in the field of IoT for a paper?

I need to know how to test the security of an implemented algorithm in the field of the IoT to state the test results in a paper. #IoT #Security #Blockchain #remix #Smart_Contract

01 July 2020 7,390 11 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View