How to bind protein to a gold nanoparticle? To rephrase, what range of pH is favorable for binding?

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Omar Gonzalez-Ortega

pH affects the charge of the protein and could also affect the zeta potential of the particles.

Asma Bahri

You can bind it by carbodiimide chemistry. You shoud make a SAM on the gold surface and then bind proteins by amine bonds. However, the protein amine groups should be positively charged. I hope I answered your question.

Emmanuele Ambrosi

It depends on which type of residue you want to use for the coupling. You can find a lot of examples (and maybe inspiration) on the book "Bioconjugate techniques" (Hermanson)