How to determine the binding efficiency of a protein to a nanoparticle?

More Hemalatha Jayabal's questions See All

How to create and validate a topology file for a small molecule using CHARMMff in GROMACS?

I have a small molecule (Ascorbic acid) that is to be used in protein simulations. Before using that in protein simulations, I wanted to validate whether my topology is correct. I obtained the...

01 February 2019 9,591 3 View

How to bind protein to a gold nanoparticle? To rephrase, what range of pH is favorable for binding?

The IEP of protein is 5.4 Size of gold nanoparticle is 5-10 nm (Synthesized by reduction of HAuCl4 using sodiumborohydride)

02 March 2018 1,546 3 View

What could be the possible reasons for observing a UV-Vis absorption peak at around 480 nm for gold nanoparticles?

Gold nanoparticles were prepared by reduction of HAuCl4 with Sodiumborohydride. The expected size of particles are around 5-10 nm.

01 February 2018 6,974 3 View

How to produce gold nanoparticles that are neutrally charged using Turkevich method?

Turkevich method produces gold nanoparticle by reduction of gold salt using citrate. The resulting nanoparticles are typically negatively charged due to citrate ions. How do we produce gold...

31 December 2017 6,607 3 View

What is the equation governing the creep response on removal of stress for a generalized voigt element?

The equation governing creep response for a generalized voigt element(On application of stress) is readily available in many literature. But, on removal of stress, the behavioral equation is not...

11 December 2017 8,780 0 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Alan F Rawle

With a Microcal ITC. See attached.

Grzegorz Celichowski

Hello

Some aspects of your question were shown in our publication. I attached the file with full text. I hope it will be helpful.

All the best from Poland

Also see:

http://www.materials-talks.com/blog/2017/10/12/isothermal-titration-calorimetry-a-hot-characterization-tool-for-biomolecular-interactions/

Hemalatha Jayabal

Thank you for your inputs. But, is there an elementary technique such as UV-Visible spectrometry or ELISA to measure the same? We do not have ITC Setups and an approximate measure would suffice since our ultimate goal is to study something else.