How to determine the binding efficiency of a protein to a nanoparticle?

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How to bind protein to a gold nanoparticle? To rephrase, what range of pH is favorable for binding?

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What could be the possible reasons for observing a UV-Vis absorption peak at around 480 nm for gold nanoparticles?

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Hello researchers Is this a random laser or just fluorescence?

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Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

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How gold procurement?

Please note it

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How gold procurement?

https://www.cleanteqwater.com/resources/case-studies/gold-mine-australia/

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Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

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Alan F Rawle

With a Microcal ITC. See attached.

Grzegorz Celichowski

Hello

Some aspects of your question were shown in our publication. I attached the file with full text. I hope it will be helpful.

All the best from Poland

Also see:

http://www.materials-talks.com/blog/2017/10/12/isothermal-titration-calorimetry-a-hot-characterization-tool-for-biomolecular-interactions/

Hemalatha Jayabal

Thank you for your inputs. But, is there an elementary technique such as UV-Visible spectrometry or ELISA to measure the same? We do not have ITC Setups and an approximate measure would suffice since our ultimate goal is to study something else.