How to assess the GLUT 10 transporter i humans?

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CyQuant cell prolif. assay - how old can it be and still work??

Hi, I have frozen two 96-well plates with cells that I need to stain using the CyQuant Cell Proliferation Assay from ThermoFisher (C7026). However, I didn't realize that we only have some very old...

06 January 2021 244 4 View

Is grain boundary scattering 'always' dominant over surface scattering in grained metal/semiconductor thin films?

Hi all, I was wondering if there is some theoretical insight in the relative contribution to the resistivity of grained thin metal or semiconductor films coming from bulk grain boundary...

18 December 2020 2,969 3 View

Can you see bands from DNA on SDS-PAGE?

If a protein sample is contaminated with nucleic acid, will these DNA/RNA run on the gel and show bands? (gradient gel, 4-20%, MW-marker: 14.4-116 kDa)

05 December 2020 9,484 3 View

Did we interpret our SDS-PAGE results correct?

This is our first time doing SDS-PAGE, and therefore we would like to hear from more experienced people if we interpret the results right. Attached is a picture of one of our gels with our...

01 December 2020 1,440 3 View

Direct Oblimin rotation and negative factor loadings?

Dear researchers, I hope you can assist me or direct me to some literature with answers. I have conducted an EFA on 22 variables which revealed 4 factors. I have used the results of the EFA to...

15 November 2020 8,078 10 View

Can someone troubleshoot my Pet28a cloning?

I am currently trying to clone a gene of 1500 bp into pet 28a by using the NdeI and XHOI restriction sites. These sites are introduced into my gene by overhang PCR. Restrictions are done for 2...

08 October 2020 5,627 7 View

Is well-being related to higher academic achievements?

Hello fellow researchers, I am writing an introduction on a new article on social marginalization in school, where I wish to refer to leading research on well-being (or school belonging) and...

13 May 2020 4,551 4 View

How to resolve CPCe run-time error "9"?

Hi When trying to export the analysis I have done of my images from CPCe I get the following error message which says "Run-time error "9": Subscript out of range". I have uploaded a screenshot...

30 April 2020 1,972 1 View

Best software to draw SEM figure?

Hi all, I am looking for the best software package to make professional looking figures when using structural equation modelling. So far I have used Amos Graphics (v. 26), but in most papers and...

24 April 2020 5,543 8 View

How to control for industry effects in a comparative case study?

I'm considering to conduct a comparative case study (following Eisenhardt 1989) and need to control for industry effects (as to be able to generalize beyond a single industry). So, I'll select...

13 October 2019 3,908 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View