How to assess the GLUT 10 transporter i humans?

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Can primary care be sustainable ? And how ?

I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...

25 July 2024 1,545 3 View

Causa of WRF-Fire crash: NaN detected in line 152?

Hello everyone, I am trying to run the Fire module of WRF 4.2.2 using real data (GFS 0.25), and I get the the following error message after running wrf.exe: Timing for processing lateral...

15 February 2024 4,695 0 View

What kind of E. coli can we use instead of XL1-Blue Competent Cells?

We had to put our pJet1.2 plasmid in a XL1-Blue Competent Cells. The result were very small colonies on a LB agar medium with ampicilin and IPTG. bands with a very low concentration were visible...

14 December 2023 354 3 View

Can leadership be defined as a (dynamic capability)?

Leadership is often defined as a microfoundation or antecedent of dynamic capabilities (DC). Yet, one could argue that 'leading' (a transformative innovation project, for example) can be...

06 August 2023 5,090 5 View

How does chlorate behave when used in oxidation of starches?

Hi, I am currently working with oxidation of starch. However, I am having issues with chlorate in my starch after it has been dried. I am using sodiumhypochlorite for oxidation. My problem is...

15 December 2022 3,637 1 View

Pigment analysis - fresh weight or dry weight?

Hi! I'm doing pigment analysis (chlorophylls and carotenoids) of seaweed, and I have some questions about the extraction and the reference system. I'm not sure if I should use fresh or dry...

02 October 2022 4,217 2 View

Anyone knows where to buy an affordable insect chill plate that runs on electricity?

Anyone knows where to buy an affordable alternative for this insect chill table, previously sold by BioQuip? It has to run for 12h constantly, so ice packs are not an option

16 June 2022 8,522 0 View

Abnormal high background in chemiluminescense assay?

Hello, we are trying to optimize a microplate assay using chemiluminescence for detection of ABO IgG2. We are experiencing abnormally high background from our reagent blanks (background >20%...

09 December 2021 6,312 1 View

How to calculate national footprint with open LCA and Exiobase?

I'm interested in developing a variety of footprints for the country of Croatia using Exiobase data in openLCA. Does anyone know if this is possible? SimaPro is probably better, but very expensive.

19 October 2021 667 4 View

Seahorse assay: when is the OCR too high so the O2 level (mmHg) gets too low (unintended hypoxia during the run)?

During Seahorse mitostress assays, we have great variance between different cell lines regarding their oxygen consumption. One cell line is tricky because at relevant confluency, the basal OCR is...

23 August 2021 3,017 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Detaching human-derived monocytes?

Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...

20 July 2024 7,922 2 View

"Hello, I am trying to find public datasets containing FTIR spectra of blood samples (both healthy and disease-related)?

These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"

17 July 2024 6,519 3 View

How to convert magnetic conductivity from SI to lattice units?

I'm simulating a magnetohydrodynamic problem of blood flow using lattice Boltzmann method in lattice units and I need to convert the magnetic conductivity to lattice units. The physical value is...

07 July 2024 5,243 0 View