18 September 2023 1 6K Report

I have mitochondrias stained with NAO probe and I would like to know how can I analyze the flow cytometry.

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Correct statistical approach for a hermit crab shell exchange experiment?

Hello, I'm planning a shell exchanging experiment with two marine, hermit crab species inside of a tank. I only have one tank available for this experiment. I plan on running 30-40 shell...

20 July 2024 5,676 3 View

Abaqus cylinder buckling riks analysis?

hi, I am doing a risk analysis of a cylindrical fuselage with windows. I have applied contact interactions between the different parts, and I have applied a unit value load to a reference point,...

20 July 2024 647 2 View

What do you think is the main advantage of cell membrane-camouflaged nanoparticles, according to your experience?

What do you think is the main advantage of cell membrane-camouflaged nanoparticles, according to your experience?

04 July 2024 6,902 2 View

How to obtain nanoparticles from microparticles?

If you prepare drug-loaded nanoparticles but only obtain microparticles (~2,000-4,000 nm), PDI~0.2, how can you reduce the size to reach ~100 nm?

02 June 2024 2,693 4 View

How to study the interaction established between two drug molecules?

How to know if two drug molecules interact with each other, the strength (and type) of interaction and the possibility to form nanoparticles?

02 June 2024 4,304 1 View

Which nanoparticle characterization step do you think is often overlooked in published research?

Curious to know your opinion on any characterization technique or step that should be looked into more detail & more often covered in order to increase the quality and robustness of...

16 May 2024 7,365 2 View

How can I solve this ABAQUS CAE error: “96 nodes are missing degree of freedoms. MPC/Equation/kinematic coupling constraints cannot be formed"?

Hello, I am making a model of an airplane fuselage, consisting of the fuselage, frames and stringers. I have made “tie” joints between the different fuselage parts, and between the frames and...

25 April 2024 8,908 1 View

Best way to prescribe periodic boundary conditions (PBC) in Abaqus/Explicit?

Hello, I'm looking to conduct simulations of RVEs (unidirectional discontinuous carbon fibers in resin matrix) utilizing the embedded finite elements technique within Abaqus. I've been successful...

09 April 2024 5,575 1 View

What can be the impact of the periodic publication of antimicrobial susceptibility patterns in analyzing the results of the samples?

The question is based on the article, “Tenets of Specimen Management in Diagnostic Microbiology” by Rajeshwar and Pathak. It seeks to understand how microbiologists can analyze specimens submitted...

09 April 2024 4,849 3 View

Is it possible to use mean-field homogenisation in Abaqus with Dynamics Implicit?

Greetings, I'm interested in whether it's possible to employ mean-field homogenization (MFH) within Abaqus alongside the Dynamics Implicit (or Explicit) solver. I'm exploring this possibility as...

26 March 2024 3,969 4 View

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Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

How to build my own lab made four point probe set up?

Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...

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Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

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Chromosomal spreads are shrunken after fluorescence in situ hybridization (FISH), why?

Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...

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Any specific peptide based ligand for mitochondrial targeted delivery of nano-drug delivery systems?

Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...

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How to enumerate mitochondria? and how to isolate them from a single cell suspension?

I am interested in analyzing the effects of some immunotherapy on the number of mitochondria in cancer cells as well as in immune cells. I would like also to isolate mitochondria to use them for...

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Can I design a probe for taqman qpcr in antisense?

I have a target of sequences for primer design for qPCR with taqman. The issue is that when I design a sense probe the probe makes dimers with forward and reverse with a very high probability at...

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Problems with designing syber-green primers for lnc_RNA GAS5?

Hi all, you know this lncRNA has 12 exons and 50 isoforms that just have similar sequences in exon 12. Coud someone recommend me primers for this exon with no need of probes?

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Can holes for pressure probes in journal bearings modify the original pressure distribution?

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Staining of isolated mitochondria tecnique?

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