I did an ELISA with supernatant of my cells that received different bacteria in different concentrations. I made the standardcurve with the standard solution that was included in the ELISA kit. What did I need to do next? How can I analyze my values that I get from my ELISA?
Hi
I have sent you a file which would be useful. I hop you find it well.
Good luck
Dear Merel, first you should plot a curve OD vs concentration (standard curve). After getting best line of your curve, you should obtain slope ve formula (y=mx+c) of your best line. Then you can calculate concentration of your samples (x in formula) by putting your OD values (y in formula). You can draw and calculate your concentrations of your samples in excel easily.
Best regards.
Hi,
You should put your plate into ELISA plate reader and watch out that the wavelengths you use correspond with the substrate. You should scan your plate using an appropriate software. Before you do the scanning, assign a template of your samples (indicate the sample code and the dilution factor) and standards (concentration). Do not forget about blank sample. The software will return the results that are "digested" for you.
Pay attention to which type of standard curve you will use - linear, semi log, log log etc.
Good luck!
Calculating and evaluating ELISA data
https://www.abcam.com/protocols/calculating-and-evaluating-elisa-data
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