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I did an ELISA with supernatant of my cells that received different bacteria in different concentrations. I made the standardcurve with the standard solution that was included in the ELISA kit....
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I used ELISA to determine the OD value and of my samples. My diluted samples gives the same OD value. I made a 10x dilution and 100x dilution. Both dilutions gives a OD value of almost 3 and my...
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I seeded Ca9-22 cells on a 96-well plate. After 24 incubation I added different bacteria in different concentrations and incubate this overnight. The next day I take out the supernatant and added...
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