I have tried 10mM Tris 1mM EDTA pH 7.4 and 10mM Tris 1mM EDTA pH 7.4 +NaCl 50mM.
My dna samples are 200bp and 1000bp long. and I treated mica with 0.1% APTES for 5min.
When I had only Tris and EDTA, the DNA was detected, but they kept moving. When NaCl was added, no DNA detected..
There's papers that looked at DNA in liquid in AFM using this buffer or environment condition in liquid with the help of APTES coated mica.. However, in these papers, the lengths of DNA used were very long (
Hi Kim,
Try adding 5-10 mM MgCl2, instead of NaCl. It should help. You can keep NaCl also in the buffer, if you want.
If you want to use divalent ions, pretreatment of mica with APTES is not necessary.
It should help.
Dear Kim,
Of the top of my head, treating mica with divalent salts such as Mg2+ may help. It should not interfere with NaCl.
Source: Article Preparation of DNA and Nucleoprotein Samples for AFM Imaging
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2997872/Let me know what you think or how it works out.
Hi Kim,
Try adding 5-10 mM MgCl2, instead of NaCl. It should help. You can keep NaCl also in the buffer, if you want.
If you want to use divalent ions, pretreatment of mica with APTES is not necessary.
It should help.
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