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Questions related from Chanwoo Kim
I have this issue with drying short DNA fragments on mica for AFM experiment. As you can see DNA in this AFM image DNA has a preference to face in a certain direction. I think it was caused by...
27 September 2022 3,273 2 View
Is there NTP/dNTP/rNTP analogue that can stall DNA polymerization at a specific base?? For example, if the template sequence is CGTTCA and I use a certain dATP analogue, can my inserted sequence...
05 October 2020 5,623 1 View
Basically, I use different concentrations and ratios of sample DNA and histone octamer as shown in the gel image. But my nucleosome band appears to be very high on the gel. This band position is...
17 January 2020 275 1 View
Well, it might sound confusing. Basically, I have several different types of DNA with distinct Watson-Crick sequences Let's say aggregation between the same DNA molecules depends on the sequence...
16 December 2019 2,936 0 View
How can i position nucleosome at a specific location in 1000bp DNA? For example, if there is widom 601 sequence, then i am aware that the nucleosome will form at widom 601. However, if i have a...
16 October 2019 4,638 10 View
I have tried 10mM Tris 1mM EDTA pH 7.4 and 10mM Tris 1mM EDTA pH 7.4 +NaCl 50mM. My dna samples are 200bp and 1000bp long. and I treated mica with 0.1% APTES for 5min. When I had only Tris and...
12 July 2019 6,037 4 View
I autoclaved my pipetman pipette. Is there any way to fix it?? The spring part and the upperpart is all damaged.
06 February 2019 4,831 2 View
I have uploaded the image of the DNA sample under Atomic Force Microscopy(AFM) I don't know why my DNA sample shows so many unspecific things. I used this protocol: Adsorption of DNA to Mica,...
02 February 2019 3,100 3 View
Hello to fellow researchers, I would like to view DNA using AFM. What I want to look at more specifically is 300nm long double stranded DNA. In AFM, samples can be visuallised either in solution...
08 January 2019 1,591 1 View
