I am conducting complement-dependent cytotoxicity assay using an IgG1 complement activating antibody and AML cells. The assay is working fine with rabbit complement, however, with normal human serum, I do not see any complement activation. I incubate the cells with the IgG1 antibody for 30 mins at 4 degC and then add complement and incubate at 37 degC for 1 hour.
Any help would be appreciated!