Dear all,

I have just purchased an aliquot of lncap from atcc, I thawed them and the result was disastrous. Briefly, these cells form big clusters and even when they adhere they maintain this morphology which seems far from the one in literature. Atcc told me that the RPMI I used (which is the same I employ for other lines) is too low in glucose, it omits sodium pyruvate and has HEPEs and sodium bicarbonate different from the one in the suggested RPMI. My ignorance, I wasn't aware that differences between RPMI from different suppliers were so important. In addition they told me I made a mistake using heat inactivated FBS as, probably, I eliminated some growth factors.

Could you kindly tell me if, in your opinion, are these requirements all mandatory for a satisfactory culture...it is so crazy. Thank you in advance