How quickly can somatic mutation alter one phenotype and one genotype to others? Why? How?

01 January 1970 0 8K Report

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Science method

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Why are reaction forces so high when I add vibration to cutting model?

Hi I am trying to model a cutting problem with a blade and comparing the results with and without ultrasonic vibration. From experimental results in our group, the use of ultrasound can...

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Best Tools for Wrist PPG Signal Analysis: Your Recommendations?

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Why diagonal terms in Abaqus stiffness matrix are infinite?

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Is it possible to combine a time-series of tree-ring widths and and a time-series of diameter at breast height measurements into one TS?

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16 May 2024 5,295 2 View

Is priori knowledge only instincts? Why?

I think so because The Random Slate Theorem

03 May 2024 3,082 7 View

How to fix driver conflicts of an ancient Skyscan 1072 micro CT with DT3157 video grabber ?

I am currently trying to get a Skyscan 1072 micro CT to work again. After the mainboard on the original PC died, we tried to set the machine up on a new computer. We managed to control the source,...

28 April 2024 381 0 View

Statistics: Is it worth using a t-test on pre and post survey data with 12 or less responses?

Is it worth using a paired t-test on pre and post survey data with 12 or less responses? Or should one rely more on the mean and standard deviation of the two data sets to evaluate the survey...

27 April 2024 8,703 8 View

What about religion, God, multi verse, etc.?

1)Religion(or at least spirituality) calms my mind, particularly, the built in belief of only one reality. God created the only reality. The afterlife is another dimension. We have free will but,...

25 April 2024 10,055 0 View

Handling BrainVision matlab files with ECG data in EEGLAB?

I have 217 preprocessed BrainVision matlab files (.mat) with ECG data in them. I've been having trouble creating a study for these. I know BioSig works for ECG data, but it doesn't seem to work...

21 April 2024 1,028 2 View

EEGLAB - how to import multiple .mat files from different folders all at once?

I have 25 folders for 25 participants, all with .mat files inside that are preprocessed. Most of them have the same amount of .mat files - a couple participants didn't finish the study, so they...

17 April 2024 1,897 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Hi, How can I design primers to obtain Deletion mutants by Site Directed Mutagenesis?

I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...

05 June 2024 9,430 1 View

How can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are there any ways to increase the rate of HDR?

Now, I am using CRISPR/Cas 9 system to make a point mutation at THBD gene. I am wondering how can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are...

30 May 2024 9,993 0 View

Which amino acids to mutate to avoid proteolysis?

We want to suppress self proteolysis of a protein. What is the strategy to modify the sequence in order to make it stable? The site is SLG - NX and SLG - AX.

25 May 2024 7,649 0 View

Can someone tell me if the following cell cluster has a pluripotent phenotype?

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20 May 2024 349 0 View

What is the most appropriate statistical test to perform when comparing the percentage of 2 cohorts that have a particular mutated gene?

I am using GraphPad prism for data visualisation and analysis. I am trying to assess if there is a statistically significant difference between the percentage of 2 cohorts (patients and cell...

17 May 2024 4,424 1 View

Which types of data can be used for lung cancer mutation detection?

I am working on a project to detect genetic mutations in lung cancer using machine learning algorithms and I am new to genetics and medical sciences. Can I use RNA as input to the algorithm for...

13 May 2024 5,726 6 View

Are they nuclear debris?

This is from an endomyocardial biopsy from a 24 year old male with clinical hypertrophic cardiomyopathy. However, no mutation is found in the related genes. What are the irregular nuclear-like...

12 May 2024 8,148 1 View