How Old Can A Bioanalyzer Chip Be For Accurate Estimation of RNA Quality?

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CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Dear Scientists, do any of You use Cresset software?

I have question about building 3DQSAR model via regression model. I cant achieve r^2 more than 0.6 for 'test set' as well as for 'training CV'-what is this?. I am wondering whether 'training set'...

17 January 2021 3,463 4 View

Why its is important the connection between wavelets and filter banks?

If multirate filter banks have been used by engeeniers before wavelets theory, why it is important and useful to know that those filter banks correspond to wavelet functions?

11 May 2020 5,895 2 View

How to teach discrete wavelet theory for undergeaduate computer scientist?

I wondering if you know texts, guides or any didactical advice that can help is to teach discrete wavelet theory to undergeaduate computer scientist

11 May 2020 3,458 3 View

Description/diagnosis vs. holotype?

The Shenzen Code in his several articles treats the way botanists should move around nomenclature. It is interesting to read all the several examples that the Code brings for each case. However,...

22 January 2020 4,817 6 View

How is it possible to detect that my UPL probe is expired or does not work?

Hello everybody. I have performed efficiency tests for primers of 12 genes with UPL probes in a Lightcycler 480 to obtain the curves with 4 dilutions of cDNA. I obtained optimal results for 10...

20 January 2020 2,431 1 View

Which are the best methods to artificially increase the size of numeric datasets ?

Hi, I'm writing a paper about Data Augmentation and I'm looking for some way of increasing the size of a dataset. I'm already aware of the techniques used for images (transformations, PCA,...

06 June 2019 8,206 6 View

Factor analysis pattern matrix value < .3 how do you interpret items that dont load 2 any factors?

If running a Factor analysis (principal axis) requesting 6 factors and suppressing value < .3 how do you interpret items that don't load onto any factors in the pattern matrix? Is this simply...

02 April 2019 4,165 2 View

Have you worked on a research project of Artificial intelligence?

At UMNG We are currently working on a Project of artificial intelligence from different prospective including ethical and legal consequences as well as artificial intelligence applied to education...

11 February 2019 1,821 3 View

What is a good digital microscope/borescope, compatible with a smartphone?

I wish to purchase a smartphone-compatible microscope, primarily for counting scales on small lizards, identifying invertebrates etc. A low magnification capability (e.g. 5 or 10x) is more...

30 December 2018 2,765 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View