Damian D Guerra

10 Questions 18 Answers 0 Followers

Questions related from Damian D Guerra

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

26 February 2021 3,604 3 View

Oops! I added the wrong secondary to my Western blot! Can I still obtain a clear signal without stripping the blot?

-I want to detect differences in protein expression via LiCoR near-infrared secondaries. -I probe with a RABBIT primary antibody, against my favorite protein. -I add the *wrong* secondary--Goat...

22 December 2017 5,303 3 View

Are Qiagen RNeasy kits suitable for extraction of yeast RNA for downstream RNAseq?

I was wondering if anyone could advise me on RNA quality. I used a Qiagen RNeasy kit to isolate RNA from yeast and an Agilent Bioanalyzer to check the quality of the RNA (see attached). Yeast...

10 June 2016 7,504 0 View

Storage recommendations for peptides?

I have immunoprecipitated my protein of interest, along with putative interacting proteins. This mixture was resolved by SDS-PAGE, and proteins were in-gel digested by trypsin. Resulting peptides...

06 June 2016 5,003 4 View

How Old Can A Bioanalyzer Chip Be For Accurate Estimation of RNA Quality?

I am interested in examining the differences in protein-coding transcripts between wild-type and a mutant. I have just purified crude RNA from yeast cells using a Qiagen RNeasy kit. By NanoDrop...

18 May 2016 1,794 2 View

Unbiased assessment of analytical ultracentrifugation (AUC) data?

I am using AUC to determine if my protein is an oligomer (more specifically, a dimer). It crystallizes as a dimer, and homologs from other species also crystallize as dimers. However, my size...

15 September 2015 1,710 4 View

Why am I getting Polyethylene glycol (PEG) contamination of mass spec sample?

I am performing mass spectrometry on an intact, denatured protein, and I am being thwarted by polyethylene glycol (PEG) contamination. I would like to know where the PEG is coming from so that I...

28 January 2015 2,279 32 View

How long after a floral dip will Agrobacterium tumifaciens continue to be capable of transforming the infected plant?

For example, if I dip an Arabidopsis plant today, will flowers that appear one week from today still be capable of being transformed? This is assuming "normative" growth conditions (ie, 20 degrees...

26 January 2015 387 13 View

I tried to synthesize GSNO (S-nitrosoglutathione) in house, but it failed to precipitate. What went wrong?

1 ml of concentrated HCl (36%) added to 10.65 ml H2O. 614 mg of glutathione was dissolved into this and was stirred on ice. 138 mg of sodium nitrite (NaNO2) were added to the acidic glutathione...

08 April 2014 7,372 7 View

Will complete Ultra Protease Inhibitor tablets irreversibly inhibit my cysteine protease?

I am purifying the SUMO-specific cysteine protease Ulp1, which I am recombinantly expressing in E. coli. My goal is to inhibit host cell proteases from cleaving my recombinant Ulp1 protease during...

25 September 2013 7,729 3 View