How to get HPLC grade water a little cheaper or does anyone know where I can find it cheaper?
While you may be able to obtain satisfactory results using double or triple distilled water that has been filtered, the detector type(s) being used and the desired goals of your method are what will determine if it will be OK. Examples: Low UV analysis will require high grade Type I, HPLC grade water. LC-MS, amino acid analysis, ion chromatography all require very high purity grades of water (please refer to the referenced article at the end for more information).
If you have access to good quality drinking water, consider the purchase of a bench-top RO system. These systems can be purchased in used condition, often at a cost of ~ 10 % of new units (you will need to purchase NEW cartridges to use them). The cartridges and filters are the consumable items so look for better pricing on those items and you should be able to setup a system which can provide "just-in-time" RO filtered water for your HPLC at a cost far below purchasing it in individual glass bottles.
Using high quality HPLC grade water with an HPLC or LC-MS system is important. Here is a link to an article on this topic which you may find helpful;
- "What type of Water Should I use for HPLC, UHPLC or LC/MS Analysis?" https://hplctips.blogspot.com/2013/10/what-type-of-water-should-i-use-for.html
HPLC grade water is available in Sigma-Aldrich, but the cost was around INR 3000 for 2.5 litre.
Instead, You can use double distilled water after filter using 0.2 micron filter. The same only we are using in our Institute without having any problems.
It depends on the HPLC detector you are using. If it a RI detector then you can double distill (ASTM I) your own lab water. If you are using a PDA/DAD then a MilliQ (or equivalent) system may be appropriate.
Yes, I agree with Bruce Neagle. The pore size of the Detector is much more important. Some of the peoples use 0.42 micron filter and somebody ll go for 0.20 micron. We should always select a filter (if having a plan to filter the water), should pass the materials having lesser dia than the dia of detector and also column for easy flow. Otherwise which would block both the things.
Yes - all that I had at my disposal back in the 1980's was glass-distilled water. I never had any problems using this, as long as I filtered it through a 0.2um pore size membrane filter before using it in any buffers. I only used HPLC water, provided by Roth or Millipore, on a couple of occasions - I was using spectrophotometric detection in the 190nm-215nm range, but it did not make enough difference to the background 'noise', for the reverse-phase separations I was using, to be worth spending the extra money on special HPLC grade water.
While you may be able to obtain satisfactory results using double or triple distilled water that has been filtered, the detector type(s) being used and the desired goals of your method are what will determine if it will be OK. Examples: Low UV analysis will require high grade Type I, HPLC grade water. LC-MS, amino acid analysis, ion chromatography all require very high purity grades of water (please refer to the referenced article at the end for more information).
If you have access to good quality drinking water, consider the purchase of a bench-top RO system. These systems can be purchased in used condition, often at a cost of ~ 10 % of new units (you will need to purchase NEW cartridges to use them). The cartridges and filters are the consumable items so look for better pricing on those items and you should be able to setup a system which can provide "just-in-time" RO filtered water for your HPLC at a cost far below purchasing it in individual glass bottles.
Using high quality HPLC grade water with an HPLC or LC-MS system is important. Here is a link to an article on this topic which you may find helpful;
- "What type of Water Should I use for HPLC, UHPLC or LC/MS Analysis?" https://hplctips.blogspot.com/2013/10/what-type-of-water-should-i-use-for.html
As I can extend your question, the final goal is to reduce the experiment or analysis cost. If so, I advise you to think about recycling of the mobile phase. Of cause, it depends on the method you use, but you can find initial information for thinking on the link below.
http://www.chromatographyonline.com/how-reduce-mobile-phase-consumption-2
Good luck.
http://www.chromatographyonline.com/how-reduce-mobile-phase-consumption-2
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