Which breast cell line makes baso-lateral membrane,tight junctions and has high polarity?

To know polarity defect (by knocking out some specific genes) I am looking for a breast cancer cell line that has high polarity, forms tight junctions and easy to transfect.

25 July 2018 9,592 3 View

How much chlorine is present in 5.75% NaOCl. How to calculate it?

I am anlyzing bleaching powder sample (5.75%) through titration which gives the amount of chlorine present. How to convert chlorine content into NaOCl?

11 December 2017 1,566 0 View

While I was using WB, I found lots of scattered band where I never expected. What is the reason?

01 February 2016 3,249 4 View

Why did my cells disconnected during the immunofluorecence procedure?

I am trying to do IF to U2OS cell line in 96 wells plate. during the procedure I lose many cells which dissociate off the plate. Does anyone has a tip how to stay with many cells at the end of the...

28 June 2015 6,856 4 View

Which is an appropriate solvent for melatonin?

04 May 2015 1,869 14 View

Which solvent can I use to dissolve melatonin for the macrophage cell line?

I want to work with the macrophage cell line with melatonin. Which solvent and how can I use it for dissolving melatonin minimizing solvents adverse effects? Can you answer elaborately with its...

04 February 2015 9,889 3 View

Which solvent can I use to dissolve melatonin for the macrophage cell line?

I want to work with the macrophage cell line with melatonin. Which solvent and how can I use it for dissolving melatonin minimizing solvents adverse effects? Can you answer elaborately with its...

01 February 2015 2,685 4 View

How can I detect or measure several proteins or mRNA by a single method simultaneously?

I would like to measure the expression of several proteins or mRNA simultaneously. I mean, can I measure by one nitrocellulose membrane (as all of my target proteins or mRNA will be present there)...

01 February 2015 9,842 6 View

I am testing a great number of recently arrived refugees/ immigrants for polio seroprotection: what is the best method and what the levels of seroprotection?

Immunity

22 October 2012 2,197 1 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

No title

02 March 2021 3,060 3 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View